Difference between revisions of "Biliverdin reductase A activity"
From Testiwiki
Juha Villman (talk | contribs) m (6 revisions: moved from Intarese wiki) |
(todo added) |
||
| Line 1: | Line 1: | ||
| + | {{variable|moderator=Marjo Niittynen}} | ||
| + | |||
| + | Graph about the causal network: [https://docs.google.com/drawings/edit?id=1c3CwuwO6UINOtDl8wduAtSI5UIrmuW_jy45FKh_zvMU&hl=en_GB] | ||
| + | |||
| + | {{todo|Lisää tähän ylläolevasta linkistä löytyvä kaavio paranneltuna sekä alkuperäinen yksilökohtainen data (uskallatko?). Voisin sen jälkeen kysyä Roger Cooken ja Patrycjan mielipidettä. Tämä on minusta Bayes-päättelyongelma, johon heillä voi olla näkemystä. |Marjo Niittynen|project=Intarese}} | ||
| + | |||
Focus: BVRA is an enzyme that reduces biliverdin to bilirubin. NADPH is consumed in the reaction. | Focus: BVRA is an enzyme that reduces biliverdin to bilirubin. NADPH is consumed in the reaction. | ||
Revision as of 04:59, 1 September 2010
This page is a variable.
The page identifier is Op_en3794 |
|---|
| Moderator:Marjo Niittynen (see all) |
|
Give your opinion to the peer rating of the content of this page. |
| Upload data
|
Graph about the causal network: [1]
TODO: {{#todo:Lisää tähän ylläolevasta linkistä löytyvä kaavio paranneltuna sekä alkuperäinen yksilökohtainen data (uskallatko?). Voisin sen jälkeen kysyä Roger Cooken ja Patrycjan mielipidettä. Tämä on minusta Bayes-päättelyongelma, johon heillä voi olla näkemystä. |Marjo Niittynen|Intarese}}
Focus: BVRA is an enzyme that reduces biliverdin to bilirubin. NADPH is consumed in the reaction.
Draft scope: There are some open questions in the right method for measuring BVRA activity. The question about blank activity will be discussed.
Variables:
- Total activity. Definition: Observed increase in absorbance (460 nm) per minute in reaction mix consisting of buffer, biliverdin, cytosol and NADPH.
- Blank. Definition: Observed increase in absorbance (460nm) per minute in reaction mix consisting of buffer, biliverdin and cytosol.
- Putatively specific activity. Definition: Total activity - blank.
Statement: Total activity is a better measure of actual BVRA activity than putatively specific activity.
Argumentation:
← Energy sources (NADPH) are available in the cytosol.
← Total activity is a specific measure of BVRA
- ← Activity ~0 if no cytosol (own observation). Even if not 0, this is the same for all samples.
- → When no cytosol, there is no BVRA
- ← Activity ~0 if no BV (own observation)
- ← It is unlikely that the reaction is unspecific
- ← Blank is large compared with total.
- ← There are large variations in blank between the samples.
