Bisphenol-A

The text on this page is taken from an equivalent page of the IEHIAS-project.

BPA is extensively employed in the production of epoxy resins and polycarbonate plastics and has estrogenic activity

Bisphenol-A as a biomarker

Sample collection and storage

Matrix:

BPA can be analyzed in blood (serum, cord, red blood cells), in breast milk, urine and feces

Kinetics:

• Food is the main uptake route of BPA
• Exposure from water and atmospheric pollution may be relevant
• In rats, BPA is rapidly absorbed, metabolized and excreted (2-3 days)
• Data from humans show BPA metabolization and excretion within 24 hours, with virtually no accumulation

Sampling conditions:

Both blood and breast milk sampled can be gathered and stored using standard procedures. Samples are stored at -20°C

Sample measurement

Analytical aspects:

• Standard measurement methods include combinations of GC, LC, MS and HPLC techniques;
• Detection limits in breast milk are reported below 1 ng/ml, and 0.6 ng/ml in fresh and seawater

Performance characteristics:

Recoveries of spiked BPA in cord blood are 65-120% and coefficients of variation are blow 15%. Intra- and inter-day variability is generally below 15%.

Validation:

Validated analytical methods are developed for cord blood, urine, and other matrices

Confounding factors:

BPA patterns may depend on food consumption patterns

Data interpretation

Concentrations reported in literature:

• Breast milk: 0.8-1.1 ng/ml
• Urine: 1.28 ng/ml
• Cord blood: ND – 4.05 ng/ml
• Blood levels: 0.41 ng/ml

Dose-response/effect relationships:

Significant increase in prostate size, decreased epididymal weight and longer anogenital distance in mice

Time trend, geographical variation, susceptibel groups:

No consistent data are available